effects of different medium on morphophysiological traits of lippia citriodora h.b.k. during in vitro culture condition

background & aim: lemon verbena (lippia citriodora h.b.k.) is a richly scented herb and has a long history as a sacred and medicinal plant. select an appropriate medium in tissue culture is a necessity for success experimental: to evaluate the effect of 10 medium of previous researches for lippia micropropagation (1- ms medium supplemented with bap (3 mgl-1) + iba (0.1 mgl-1) + activated charcoal (1 gl-1) 2- ms medium supplemented with bap (1 mgl-1) + iba (0.5 mgl-1) + activated charcoal (1 gl-1) 3- 1/2ms 4- ms medium supplemented with bap (5 mgl-1) 5- ms medium supplemented with bap (1 mgl-1) 6- ms medium supplemented with activated charcoal (1 gl-1) 7- ms medium supplemented with bap (1 mgl-1) + activated charcoal (1 gl-1) 8- ms medium supplemented with bap (1 mgl-1) + agno3 (5 mgl-1) 9- ms medium supplemented with agno3 (5 mgl-1) 10- ms (control)), an experiment was conducted as a completely randomized design (crd) with three replicates and three explants per replication.  results & discussion: the results showed positive effects for bap (5 mgl-1) and somewhat active charcoal, but contrary to predictions, agno3 had no effect on reducing senescence and leaf necrosis. recommended applications/industries: therefore, based on the finding, the four medium (ms medium supplemented with bap (5 mgl-1)) with the highest rates of shoot fresh weight, leaf number, internodes number, shoot number, root fresh weight, callus diameter and root dry weight can be introduced as a suitable medium of tissue culture in this experimental conditions.